chang apache root directory

I am having problems changing the Permissions on the "username.conf" file in the /etc/apache2/users/ folder. It need to have the Permission of -rw-r--r-- 1 root wheel 298 Jun 28 16:47 username.conf

But I am not being successful at it. I have tried with sudo chmod 644 username.conf; and it didn't change. I am using the tutorial http://ift.tt/1HgFdxM

When I check the permission of the file, it's -rw-r--r-- 1 root wheel 155 Jan 23 23:30 Owner.conf

When I navigate to http://localhost/~Owner/ , I get 404 Not found.